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Last active February 22, 2026 04:41
WES Test Report: nf-core/rnaseq via Sapporo on macOS (Apple Silicon)

WES Test Report: nf-core/rnaseq via Sapporo

Environment

  • Host: macOS (Apple Silicon / arm64), Darwin 24.5.0
  • Docker Desktop: v29.2.1, API v1.53 (minimum API v1.44)
  • Sapporo WES: sapporo-wes-2.1.0 (ghcr.io/sapporo-wes/sapporo-service:latest)
  • Nextflow: 25.10.4 (via nextflow/nextflow:25.10.4 container)
  • Pipeline: nf-core/rnaseq (test profile)
  • Date: 2026-02-22
require 'nokogiri'
require 'csv'
require 'open-uri'
urls = [
'https://www.iscb.org/cms_addon/conferences/ismbeccb2025/posters.php?track=BOSC&session=A#search',
'https://www.iscb.org/cms_addon/conferences/ismbeccb2025/posters.php?track=BOSC&session=B#search',
'https://www.iscb.org/cms_addon/conferences/ismbeccb2025/posters.php?track=BOSC&session=E#search'
]
reference:
class: File
path: Homo_sapiens_assembly38.fasta
format: http://edamontology.org/format_1929
sortsam_max_records_in_ram: 5000000
sortsam_java_options: -XX:-UseContainerSupport -Xmx8g -Xms8g
gatk4_MarkDuplicates_java_option: -XX:-UseContainerSupport -Xmx32g -Xms32g
gatk4_BaseRecalibrator_java_options: -XX:-UseContainerSupport -Xmx8g -Xms8g
gatk4_ApplyBQSR_java_options: -XX:-UseContainerSupport -Xmx8g -Xms8g
gatk4_HaplotypeCaller_java_options: -XX:-UseContainerSupport -Xmx8g -Xms8g
reference: # type "File"
class: File
format: http://edamontology.org/format_1929
location: http://data.dbcls.jp/~inutano/human-reseq/test/data/human-reseq-test-data/hs37d5.release-20190328/hs37d5.fa
secondaryFiles:
- class: File
location: http://data.dbcls.jp/~inutano/human-reseq/test/data/human-reseq-test-data/hs37d5.release-20190328/hs37d5.dict
- class: File
location: http://data.dbcls.jp/~inutano/human-reseq/test/data/human-reseq-test-data/hs37d5.release-20190328/hs37d5.fa.amb
- class: File
{
"$graph": [
{
"class": "CommandLineTool",
"id": "#bwa-mem-SE.cwl",
"label": "bwa-mem-SE-0.7.12",
"hints": [
{
"class": "DockerRequirement",
"dockerPull": "biocontainers/bwa:v0.7.12_cv3"
{
"stanzas": [
"https://togostanza.github.io/metastanza/hash-table.js",
"https://togostanza.github.io/metastanza/barchart.js"
],
"templates": {
"variant": "https://raw.githubusercontent.com/dbcls/togosite/fix-hbs-render/config/togosite-human/templates/variant.hbs",
"gene": "https://raw.githubusercontent.com/dbcls/togosite/fix-hbs-render/config/togosite-human/templates/gene.hbs",
"protein": "https://raw.githubusercontent.com/dbcls/togosite/fix-hbs-render/config/togosite-human/templates/protein.hbs",
"interaction": "https://raw.githubusercontent.com/dbcls/togosite/fix-hbs-render/config/togosite-human/templates/interaction.hbs",
@base <http://schema.org/> .
@prefix rdf: <http://www.w3.org/1999/02/22-rdf-syntax-ns#> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix provo: <http://www.w3.org/ns/prov#> .
<http://identifiers.org/biosample/SAMN03765156>
<mainEntity> [
<additionalProperty> [
<name> "cell line" ;
<value> "HeLa-229" ;
ENV DEBIAN_VERSION=buster-20200327
ENV ODGI_COMMIT=5e18490156f08368dae0b25a69e0cf5efa5427ca
ENV SPODGI_COMMIT=84a1af531e5381216eeb5035e284167ed886da21
FROM debian:${DEBIAN_VERSION} as odgi_build
WORKDIR "/tmp"
RUN DEBIAN_FRONTEND=noninteractive \
&& apt-get update -y \
&& apt-get install -y --no-install-recommends \
build-essential \
Sat Feb 08 2020
01:57:36 { Loading plugin 1: Type `plain', file `geos' in `../hosting'
01:57:36 plain version 1.0.3230 from OpenLink Software
01:57:36 GEOS plugin based on Geometry Engine Open Source library from Open Source Geospatial Foundation
01:57:36 SUCCESS plugin 1: loaded from ../hosting/geos.so }
01:57:36 { Loading plugin 2: Type `plain', file `im' in `../hosting'
01:57:36 IM version 0.62 from OpenLink Software
01:57:36 Support functions for Image Magick 6.9.9
01:57:36 SUCCESS plugin 2: loaded from ../hosting/im.so }
require 'yaml'
require 'json'
require 'pp'
module CWL
class Workflow
def initialize(fpath)
@workflow = YAML.load(open(fpath))
end